Analytical Studies of New Azo dye and the Diminished in their Biological Activity by Complexation with Zinc

doi:https://doi.org/10.5281/zenodo.16652335

Biomedicine and Chemical Sciences

2025, Volume 4, Issue 3 : 88-96 doi: https://doi.org/10.5281/zenodo.16652335

Research Article

Analytical Studies of New Azo dye and the Diminished in their Biological Activity by Complexation with Zinc

Ali Kamil Mohsin

Hanan M. Ali

DOI : https://doi.org/10.5281/zenodo.16652335

Received

April 28, 2025

Revised

June 15, 2025

Accepted

June 27, 2025

Published

July 1, 2025

Abstract

Azo dye (1), that characterized (Z)-5-amino-2-(((4-(5-(p-tolyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl) phenyl) sulfonyl) diazenyl) phenol) was prepared. Then characterized using m.p., IR, UV-visible, mass spectrum and elemental analysis (CHN). Analytical studies approved on this dye; which showed high solubility in ethanol and gave three isopiestic points, that when studying the pH effect in a range of buffer solutions. The protonation constants pKb1 and pKb2 of nitrogen atom and the ionization constant pKa of OH-group were equal to 3.4, 7.26 and 10.5 respectively. These results indicated the suggested ionization scheme in acidic and basic media. However, due to recommend the new azo dye as novel medicine or chemical sanitizer especially in dual infection. the complex of azo dye (1) with Zn was decreased the biological activity of dye.

Keywords

Azo dye

Biological activity

Elemental analysis

Haemolysis effects

Ionization constants

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