Biomedicine and Chemical Sciences
2023, Volume 2, Issue 1 : 39-43 doi: https://doi.org/10.48112/bcs.v2i1.334
Research Article
Investigation of Tritrichomonas Foetus in Cattle Using Different Methods in Basrah City – Iraq
 ,
 ,
Received
Oct. 26, 2022
Revised
Nov. 12, 2022
Accepted
Nov. 13, 2022
Published
Jan. 1, 2023
Abstract

Tritrichomonas foetus causes a venereal disease in cattle called bovine trichomoniasis. T. foetus lives in the reproductive tract of the cow, the surface of the penis and prepuce of the bull and causing early fetal loss and sometimes late term abortions. It can also extend breeding / calving season. In this study, 155 cows of different ages (2-6 years old) and with a variety of clinical signs (repeat breeders, abortions, and discharge only) were examined from different areas in Basrah Province, Iraq from November 2020 to July 2021, and 75 samples collected from healthy animals (without any symptom). The infection rate was detected in wet mount techniques found fewer positive findings than in pouch techniques, with 12 (40%) recorded and in pouch systems, 30 (100%) recorded while in the culture 24(80 %), Giemsa-stained smears 19(63.3%) and Acridine orange was 20 (66.6%). The present study improved that cows more than three years are more susceptible to be infected than cows less than three years. The study concluded that if no control method is applied in Basrah, there is a serious risk of spreading Trichomonas foetus.

Keywords
REFERENCES
  1. Alobaidii, W. A., Alobaidii, Q. T., & Hassan, S. D. (2021). Detection of Trichomoniasis in cattle in Nineveh province. Iraqi Journal of Veterinary Sciences, 35(2), 287-290. http://dx.doi.org/10.33899/ijvs.2020.126790.1380
  2. Bauer J.D., Ackermann P.G. and Toro G. (1974). Clinical laboratory method .8th ed., The C.V. Mosby Company, Saint Louis, P:50-51,85- 86 .
  3. BonDurant, R. H., Anderson, M. L., Blanchard, P., Hird, D., Danaye-Elmi, C., Palmer, C., ... & Weigler, B. J. (1990). Prevalence of trichomoniasis among California beef herds. Journal of the American Veterinary Medical Association, 196(10), 1590-1593. https://europepmc.org/article/med/2347750
  4. Bryan, L. A., Campbell, J. R., & Gajadhar, A. A. (1999). Effects of temperature on the survival of Tritrichomonas foetus in transport, Diamond's and InPouch TF media. Veterinary Record, 144(9), 227-232. https://doi.org/10.1136/vr.144.9.227
  5. Campero, C. M., Dubra, C. R., Bolondi, A., Cacciato, C., Cobo, E., Perez, S., ... & BonDurant, R. H. (2003). Two-step (culture and PCR) diagnostic approach for differentiation of non-T. foetus trichomonads from genitalia of virgin beef bulls in Argentina. Veterinary parasitology, 112(3), 167-175. https://doi.org/10.1016/S0304-4017(02)00423-5
  6. Cobo, E. R., Favetto, P. H., Lane, V. M., Friend, A., VanHooser, K., Mitchell, J., & BonDurant, R. H. (2007). Sensitivity and specificity of culture and PCR of smegma samples of bulls experimentally infected with Tritrichomonas foetus. Theriogenology, 68(6), 853-860. https://doi.org/10.1016/j.theriogenology.2007.06.019
  7. Corbeil, L. B., Anderson, M. L., Corbeil, R. R., Eddow, J. M., & BonDurant, R. H. (1998). Female reproductive tract immunity in bovine trichomoniasis. American Journal of Reproductive Immunology, 39(3), 189-198. https://doi.org/10.1111/j.1600-0897.1998.tb00353.x
  8. Dahab, M. M., Koko, W. S., Osman, E. E., & Hilali, A. H. M. (2012). Prevalence and transmission of Trichomonas vaginalis infection among women in Khartoum State, Sudan. Journal of Public health and Epidemiology, 4(2), 34-38.
  9. Diamond, L. S. (1957). The establishment of various trichomonads of animals and man in axenic cultures. The Journal of parasitology, 43(4), 488-490. https://doi.org/10.2307/3274682
  10. Gregory, M. W., Elles, B., & Redwood, D. W. (1990). Comparison of sampling methods for the detection of Trichomonas fetus. Journal of veterinary research, 4, 1430-149.
  11. J Hassan, B. (2013). Detection of bovine trichomoniasis of bulls in Basrah slaughterhouse. Basrah Journal of Veterinary Research, 12(1), 98-103. http://dx.doi.org/10.33762/bvetr.2013.76192
  12. Jebara, K. B., Cáceres, P., Berlingieri, F., & Weber-Vintzel, L. (2012). Ten years’ work on the world organisation for animal health (OIE) worldwide animal disease notification system. Preventive veterinary medicine, 107(3-4), 149-159. https://doi.org/10.1016/j.prevetmed.2012.08.008
  13. Kittel, D. R., Campero, C., Van Hoosear, K. A., Rhyan, J. C., & BonDurant, R. H. (1998). Comparison of diagnostic methods for detection of active infection with Tritrichomonas foetus in beef heifers. Journal of the American Veterinary Medical Association, 213(4), 519-522. https://europepmc.org/article/med/9713536
  14. Krieger, J. N., Verdon, M., Siegel, N., & Holmes, K. K. (1993). Natural history of urogenital trichomoniasis in men. The Journal of urology, 149(6), 1455-1458. https://doi.org/10.1016/S0022-5347(17)36414-5
  15. Lun, Z. R., & Gajadhar, A. A. (1999). A simple and rapid method for staining Tritrichomonas foetus and Trichomonas vaginalis. Journal of Veterinary Diagnostic Investigation, 11(5), 471-474.
  16. Murtaza S., Raza M.A., Akhtar M. S., Ayaz M. M., Farooq M. A. A., Ali M. and Ali F. (2015). Predominance of Bovine Trichomoniasis in Multan District, Adjacent Areas, Pakistan. J. Vet. Anim. Sci., 5(1):50.
  17. Nasir, M. A., Al-Idreesi, S. R., & Al-Masoudi, W. A. (2022). Comparison of Different Techniques for the Diagnosis of Trichomonas Vaginalis Infection in Females at Reproductive Age. Pakistan Journal of Medical & Health Sciences, 16(07), 451-451. https://doi.org/10.53350/pjmhs22167451
  18. Parker, S., Campbell, J., & Gajadhar, A. (2003). Comparison of the diagnostic sensitivity of a commercially available culture kit and a diagnostic culture test using Diamond's media for diagnosing Tritrichomonas foetus in bulls. Journal of veterinary diagnostic investigation, 15(5), 460-465. https://doi.org/10.1177/104063870301500510
  19. Radonjic, I. V., Dzamic, A. M., Mitrovic, S. M., Arsenijevic, V. S. A., Popadic, D. M., & Zec, I. F. K. (2006). Diagnosis of Trichomonas vaginalis infection: the sensitivities and specificities of microscopy, culture and PCR assay. European Journal of Obstetrics & Gynecology and Reproductive Biology, 126(1), 116-120. https://doi.org/10.1016/j.ejogrb.2005.07.033
  20. Rae, D. O., Crews, J. E., Greiner, E. C., & Donovan, G. A. (2004). Epidemiology of Tritrichomonas foetus in beef bull populations in Florida. Theriogenology, 61(4), 605-618. https://doi.org/10.1016/S0093-691X(03)00236-X
  21. Saba, A. M. (2012). The usage of In-Pouch TV culture system as a method for Trichomonas vaginalis detection in Baghdad/AL-Karkh (Doctoral dissertation, MSc thesis. College of Science. University of Baghdad).
  22. Sallu, F., Asare, D. A., Aseno, N. T., Essel-Cobbinah, D., & Emikpe, B. O. (2022). Detection of Tritrichomonas foetus in cattle at the Kumasi abattoir. Nigerian Journal of Parasitology, 43(1), 34-40.
  23. Taylor, M. A., Marshall, R. N., & Stack, M. (1994). Morphological differentiation of Tritrichomonas foetus from other protozoa of the bovine reproductive tract. British Veterinary Journal, 150(1), 73-80. https://doi.org/10.1016/S0007-1935(05)80098-3
  24. Waters, K., & Gard, J. (2021). Infectious Agents: Tritrichomonas. Bovine Reproduction, 725-732. https://doi.org/10.1002/9781119602484.ch58
  25. Yao, C. (2013). Diagnosis of Tritrichomonas foetus-infected bulls, an ultimate approach to eradicate bovine trichomoniasis in US cattle?. Journal of medical microbiology, 62(1), 1-9. https://doi.org/10.1099/jmm.0.047365-0
  26. Youngquist, R. S., & Threlfall, W. R. (2006). Current therapy in large animal theriogenology. Elsevier Health Sciences.
Recommended Articles
Original Article Open Access
A FRAMEWORK OF MONOCLONAL ANTIBODIES AND RELATED PRODUCTION ENGINEERING
2025, Volume 4, Issue 4 : 26-34
Research Article Open Access
Spectroscopic and Thermal Studies of Some Palladium(II) Complexes with 2-amino-4-(4-subsistuted phenyl)thiazole Derivatives
2022, Volume 1, Issue 2 : 78-82
DOI: https://doi.org/10.48112/bcs.v1i2.104
Research Article Open Access
The Role of Vitamin D and Zinc In Facing COVID-19 Injury
2022, Volume 1, Issue 1 : 6-10
DOI: https://doi.org/10.48112/bcs.v1i1.76
Research Article Open Access
Design and Synthesis Ligands Tetradents Substituted with Halogenes in α- Position and Conjugation with Riboflavin (Bioconjugates)
2022, Volume 1, Issue 2 : 47-56
DOI: https://doi.org/10.48112/bcs.v1i2.85
Biomedicine and Chemical Sciences journal thumbnail
Volume 2, Issue 1
Citations
1 Views
0 Downloads
Share this article
License
Copyright (c) Biomedicine and Chemical Sciences
Creative Commons Attribution License Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
All papers should be submitted electronically. All submitted manuscripts must be original work that is not under submission at another journal or under consideration for publication in another form, such as a monograph or chapter of a book. Authors of submitted papers are obligated not to submit their paper for publication elsewhere until an editorial decision is rendered on their submission. Further, authors of accepted papers are prohibited from publishing the results in other publications that appear before the paper is published in the Journal unless they receive approval for doing so from the Editor-In-Chief.
Biomed. Chem. Sci. open access articles are licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. This license lets the audience to give appropriate credit, provide a link to the license, and indicate if changes were made and if they remix, transform, or build upon the material, they must distribute contributions under the same license as the original.
Biomedicine and Chemical Sciences Logo
Biomedicine and Chemical Sciences
About Us
Biomedicine and Chemical Sciences (BCS), an international journal, publishes double blind peer-reviewed full-length, original papers, reviews or letters. BCS covers the latest developments in various fields of biomedicine such as cardiology, immunology, genetics, environmental health, neurology, oncology and toxicology
Support
Follow Us
facebook twitter linkedin mendeley research-gate
© Copyright Biomedicine and Chemical Sciences (BCS). All Rights Reserved.