Isolation, Identification and Antibacterial Activity of Alkaloid Compound N-Methylcytisine from Cyanobacterium Hapalosiphon Aureus

doi:https://doi.org/10.48112/bcs.v2i2.447

Biomedicine and Chemical Sciences

2023, Volume 2, Issue 2 : 119-124 doi: https://doi.org/10.48112/bcs.v2i2.447

Research Article

Isolation, Identification and Antibacterial Activity of Alkaloid Compound N-Methylcytisine from Cyanobacterium Hapalosiphon Aureus

Nida J. M. Al-Mousawi

Iqbal J. Al-Assadi

Moussa J. Al-Aarajy

DOI : https://doi.org/10.48112/bcs.v2i2.447

Received

Jan. 22, 2023

Revised

Feb. 4, 2023

Accepted

Feb. 14, 2023

Published

April 1, 2023

Abstract

In this study, one species of algae, belongated to cyanophyta : Hapalosiphon aureus west and west 1897 were isolated, identified and purified. The extracts of algal isolates were testing to clarify their ability on growth inhibition on each of gram +ve and gram –ve bacteria E.coli & S. aureus, filamentous fungi A. fumigatus, and yeasts C.albican. The extracted alkaloid of species H.aureus showed higher inhibition activity. In our literature survey, the alkaloid compound analog N- methylcytisine was isolated for the first time from the H.aureus. This compound was characterized using Thin Layer Chromatography (TLC), Ultra Violet Spectrum (UV), Infrared Spectrum (IR), Proton Nuclear Magnetic Resonance Spectroscopy (¹HNMR), Melting Point and Solubility in organic and inorganic solvents. The biological activity of this alkaloid was determined using the Minimal Inhibitory Concentration (MIC) against six bacterial isolates. The results showed inhibition in 33.3% growth of bacteria on 150 μg/ml concentration level, while the MIC against six fungi isolates showed inhibition of growth 33.3% at 50 μg/ml compared to standard antibiotic. The median lethal dose (LD₅₀) showed no toxicity, no cytotoxicity and no effect on human red blood cells for 18 hours.

Keywords

Antibacterial activity

Extract alkaloid

Cyanobacteria

H.aureus

N-methylcytisin

REFERENCES

Al Naim, H. M., & El Semary, N. (2022). Laser Treatment Increases the Antimicrobial Efficacy of Cyanobacterial Extracts against Staphylococcus aureus (SA) and Methicillin-resistant Staphylococcus aureus (MRSA). International Journal of Environmental Research and Public Health, 19(20), 13305. https://doi.org/10.3390/ijerph192013305
Abdulredha, D. S., AlMousawi, N. J., & Azeez, N. M. (2021). Using Blue-Green Algae Hapalosiphon Sp. & Green Algae Scenedesmus Spp. In Reducing Organic Pollutants from Wastewater. Annals of the Romanian Society for Cell Biology, 25(6), 8647-8653. https://annalsofrscb.ro/index.php/journal/article/view/7106
Al-Aarajy, M. J. (2012). Effect of ethanolic and oil extracted from some Cyanobacteri on the growth of bacteria. yeast and filamentous fungi. basrah journal of science, 30(2B Environmental Conference). https://www.iasj.net/iasj/article/69221
Al-Hejuje, M. M. (2008). Effect of some heavy metals ions on the chlorophyll a pigment of Nostoc linkia and Hapalosiphon aureus. Marsh Bulletin, 3(2), 136-146.
Becher, P. G., Beuchat, J., Gademann, K., & Jüttner, F. (2005). Nostocarboline: isolation and synthesis of a new cholinesterase inhibitor from Nostoc 78-12A. Journal of natural products, 68(12), 1793-1795. https://doi.org/10.1021/np050312l
Biondi, N., Piccardi, R., Margheri, M. C., Rodolfi, L., Smith, G. D., & Tredici, M. R. (2004). Evaluation of Nostoc strain ATCC 53789 as a potential source of natural pesticides. Applied and environmental microbiology, 70(6), 3313-3320. https://doi.org/10.1128/AEM.70.6.3313-3320.2004
Chen, X., Smith, G. D., & Waring, P. (2003). Human cancer cell (Jurkat) killing by the cyanobacterial metabolite calothrixin A. Journal of applied phycology, 15, 269-277. https://doi.org/10.1023/A:1025134106985
Ma, S. C., Du, J., But, P. P. H., Deng, X. L., Zhang, Y. W., Ooi, V. E. C., ... & Lee, S. F. (2002). Antiviral Chinese medicinal herbs against respiratory syncytial virus. Journal of ethnopharmacology, 79(2), 205-211. https://doi.org/10.1016/S0378-8741(01)00389-0
Chetsumon, A., Umeda, F., Maeda, I., Yagi, K., Mizoguchi, T., & Miura, Y. (1998). Broad spectrum and mode of action of an antibiotic produced by Scytonema sp. TISTR 8208 in a seaweed-type bioreactor. In Biotechnology for Fuels and Chemicals: Proceedings of the Nineteenth Symposium on Biotechnology for Fuels and Chemicals Held May 4-8. 1997, at Colorado Springs, Colorado (pp. 249-256). Humana Press. https://doi.org/10.1007/978-1-4612-1814-2_24
De Morais, M. G., Vaz, B. S., De Morais, E. G., & Costa, J. A. V. (2015). Biologically active metabolites synthesized by microalgae. Biomed Research International.
Dasgupta, C. N. (2015). Algae as a source of phycocyanin and other industrially important pigments. Algal biorefinery: An integrated approach, 253-276. https://doi.org/10.1007/978-3-319-22813-6_12
El-Sheekh, M. M., Osman, M. E., Dyab, M. A., & Amer, M. S. (2006). Production and characterization of antimicrobial active substance from the cyanobacterium Nostoc muscorum. Environmental toxicology and pharmacology, 21(1), 42-50. https://doi.org/10.1016/j.etap.2005.06.006
Fieser, L. F., & Williamson, K. L. (1968). Organic experiments. Lexington: Raytheon Education Company.
Fu, W., Nelson, D. R., Yi, Z., Xu, M., Khraiwesh, B., Jijakli, K., ... & Salehi-Ashtiani, K. (2017). Bioactive compounds from microalgae: Current development and prospects. Studies in natural products chemistry, 54, 199-225. https://doi.org/10.1016/B978-0-444-63929-5.00006-1
Ghasemi, Y., Tabatabaei, Y. S., Zarini, G., Shokravi, S., & Soltani, N. (2003). Antifungal and antibacterial activity of paddy-fields cyanobacteria from the north of Iran.
Khalaf, A. K. (2020). In Vitro Activity of Methanol Extract of Microalgae Hapalosiphon aureus Against Trichomonas vaginalis. In Vitro, 7(2).
Al-Mousawi, N. J., Al-Assadi, I. J., & Al-Aarajy, M. J. (2023). Isolation, Identification and Antibacterial Activity of Alkaloid Compound N-Methylcytisine from Cyanobacterium Hapalosiphon Aureus. Biomedicine and Chemical Sciences, 2(2). https://doi.org/10.48112/bcs.v2i2.447
Nair, M. G., Putnam, A. R., Mishra, S. K., Mulks, M. H., Taft, W. H., Keller, J. E., ... & Lynn, D. G. (1989). Faeriefungin: a New board-spectrum antibiotic from Streptomyces griseus var. autotrophicus. Journal of natural products, 52(4), 797-809. https://doi.org/10.1021/np50064a022
Wayne, P. A. (2006). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A7. Clinical and Laboratory Standards Institute.
Rickards, R. W., Rothschild, J. M., Willis, A. C., de Chazal, N. M., Kirk, J., Kirk, K., ... & Smith, G. D. (1999). Calothrixins A and B, novel pentacyclic metabolites from Calothrix cyanobacteria with potent activity against malaria parasites and human cancer cells. Tetrahedron, 55(47), 13513-13520. https://doi.org/10.1016/S0040-4020(99)00833-9
Hellebust, J. A. (1985). Handbook of phycological methods: volume 4: ecological field methods: macroalgae (Vol. 4). Cambridge University Press.
Walton, K., & Berry, J. P. (2016). Indole alkaloids of the Stigonematales (Cyanophyta): Chemical diversity, biosynthesis and biological activity. Marine Drugs, 14(4), 73. https://doi.org/10.3390/md14040073

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