Standardization and Characterization of Essential Oil of Patchouli Stem (Pogostemon cablin Benth.) By Chromatography-Mass Spectrometry (GC-MS) Method

doi:https://doi.org/10.5281/zenodo.15776248

Biomedicine and Chemical Sciences

2024, Volume 3, Issue 3 : 83-92 doi: https://doi.org/10.5281/zenodo.15776248

Research Article

Standardization and Characterization of Essential Oil of Patchouli Stem (Pogostemon cablin Benth.) By Chromatography-Mass Spectrometry (GC-MS) Method

Aktsar Roskiana Ahmad

Rafika Haerunnisasaleh

Virsa Handayani

DOI : https://doi.org/10.5281/zenodo.15776248

Received

Aug. 18, 2022

Revised

April 20, 2024

Accepted

May 25, 2024

Published

July 1, 2024

Abstract

Patchouli (Pogostemon cablin Benth.) is one kind of the plants producing essential oil that can be used in the treatment, because the content on the patchouli oil is seskuiterpen, cytotoxic chalcones, and antimutagenik. This study aimed to establish the standardization of crude drugs and characterization of essential oil of patchouli stem by GC-MS. The research carried out includes the determination of specific and non-specific parameters, as well as the characterization of essential oil by GC-MS. The standardization result include the non-specific parameters including identity test, that is, patchouli stem botanicals, botanical organoleptic test forming dry powder, khaki, distinctive smell, bitter taste, water soluble extract content 0f 5,9891%, levels of ethanol soluble extract 0f 8,0578% and positive compounds identification containing alkaloids, flavonoids, terpenoids, essential oils as well as specific parameters, that is, drying shrinkage of 0,8281%, moisture content of 1,7968%, total ash content of 5,7730%, and acid insoluble ash content of 0,3459%. The result of GC-MS characterization of essential oils derived from patchouli stem by stahl distillation shows five main components, namely benzaldehyde, 2,4-dimethyl, phenol,2,4-Bis91,1-Dimethylethyl1,6-Methanonaphthalen-1(2H)-OL, Octahydro-4,8A,9,9-Tetramethyl, octadecanooic acid, ethyl ester and hexadecanoic acid, ethyl ester.

Keywords

Patchouli oil

Simplicia standardization parammeters.

REFERENCES

Agusta, A. (2000). Minyak atsiri tumbuhan tropika Indonesia. Penerbit ITB.
Arifin, H., Anggraini, N., Handayani, D., & Rasyid, R. (2006). Standarisasi ekstrak etanol daun Eugenia cumini Merr. J. Sains Tek. Far, 11(2), 88-93.
Farnsworth, N. R. (1966). Biological and phytochemical screening of plants. Journal of Pharmaceutical Sciences, 55(3), 225-276. https://doi.org/10.1002/jps.2600550302
Gunawan, D., & Mulyani, S. (2004). Ilmu obat alam (farmakognosi). Penebar Swadaya, Jakarta, 81, 83.
Harbone, J. B. (1987). Metode Fitokimia, Edisi ke dua. ITB. Bandung.
Hernawati, N. S., Budiarti, A., & Mahfud, M. (2012). Proses pengambilan minyak atsiri dari daun nilam dengan pemanfaatan gelombang mikro (microwave). Jurnal Teknik ITS, 1(1), F25-F29. http://dx.doi.org/10.12962/j23373539.v1i1.453
Liana, M., Fitrianingsih, S. P., & Mulqie, L. (2015). Karakterisasi Simplisia Dan Ekstrak Etanol Jamur Kuping (Auricularia polytricha (Mont.) Sacc.). Jurnal Prosiding Unisba, hal, 267-273.
Lumbanraja, L. B. (2009). Skrining Fitokimia dan Uji Efek Antiinflamasi Ekstrak Etanol Daun Tempuyung (Sonchus arvensis L.) terhadap Radang pada Tikus. Skripsi. Fakultas Farmasi, Universitas Sumatera Utara, 83.
Marliana, S. D., Suryanti, V., & Suyono, S. (2005). Skrining fitokimia dan analisis kromatografi lapis tipis komponen kimia buah labu siam (Sechium edule Jacq. Swartz.) dalam ekstrak etanol. Biofarmasi, 3(1), 26-31.
Marlinda, M., Sangi, M. S., & Wuntu, A. D. (2012). Analisis senyawa metabolit sekunder dan uji toksisitas ekstrak etanol biji buah alpukat (Persea americana Mill.). Jurnal Mipa, 1(1), 24-28. https://doi.org/10.35799/jm.1.1.2012.427
Maulida, R., & Guntarti, A. (2015). Pengaruh Ukuran Partikel Beras Hitam (Oryza Sativa L.) Terhadap Rendemen Ekstrak Dan Kandungan Total Antosianin.[Influence of black rice particle size (Oryza Sativa L.) against rendement extract and total content of antosianin]. J Pharm, 5(1), 9-16.
Miyazawa, M., Okuno, Y., Nakamura, S. I., & Kosaka, H. (2000). Antimutagenic activity of flavonoids from Pogostemon cablin. Journal of Agricultural and Food Chemistry, 48(3), 642-647. https://doi.org/10.1021/jf990160y
Nickavar, B., Mojab, F., & Dolat-Abadi, R. (2005). Analysis of the essential oils of two Thymus species from Iran. Food Chemistry, 90(4), 609-611. https://doi.org/10.1016/j.foodchem.2004.04.020
Nuryani, Y. (2006). Karakteristik empat aksesi nilam. Indonesian Ministry of Agriculture.
Soetarno, S., & Soediro, I. S. (1997). Standarisasi Mutu Simplisia dan Ekstrak Bahan Obat Tradisional. Presidium Temu Ilmiah Nasional Bidang Farmasi.
Spesifik, S. P. N., & Salamah, N. (2013). Standarisasi Parameter Non Spesifik Dan Perbandingan Kadar Kurkumin Ekstrak Etanol Dan Ekstrak Terpurifikasi Rimpang Kunyit Standardization Of Non Specific. Jurnal Ilmiah Kefarmasian, 3(1), 21-30.
Sukmajaya, P., & Putra, B. (2012). Analisis Kandungan Minyak Atsiri Daun Tenggulun (Protium Javanicum Burm. F.) Dengan Metode Kromatografi Gas-Spektroskopi Massa. Jurnal Kimia, 6(2), 155-162.
Sulaksono, S., Fitrianingsih, S. P., & Yuniarni, U. (2015). Karakterisasi simplisia dan ekstrak etanol buah salak (Salacca zalacca (Gaertner) Voss). Prosiding KNMSA, 317-320.
Syauqiah, I., Mirwan, A., Sulaiman, A., & Nurandini, D. (2008). Analisis pengaruh lama penyulingan dan komposisi bahan baku terhadap rendemen dan mutu minyak atsiri dari daun dan batang nilam. Infoteknik, 9(1), 21-30.
Zaenab, Gunarti F, Witasari, H, A, Edityaningrum, C, A, Mustafa & Murrukmihadi, M. (2016). Penetapan Parameter Standarisasi Non Spesifik Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi L.). Prosiding Rakernas dan Pertemuan Ilmiah Tahunan Ikatan Apoteker Indonesia.
Zaimah, S. (2014). Pengujian kualitas dan komposisi kimia minyak nilam (Pogostemon cablin benth) setelah penyimpanan. Indonesian Journal of Chemical Research, 1-9. https://doi.org/10.20885/ijcr.vol1.iss2.art1
Zhao, Z., Lu, J., Leung, K., Chan, C. L., & Jiang, Z. H. (2005). Determination of patchoulic alcohol in herba pogostemonis by GC-MS-MS. Chemical and Pharmaceutical Bulletin, 53(7), 856-860. https://doi.org/10.1248/cpb.53.856

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